Specimen identification is a critical part of outbreak investigations. Once the causative agent is known through specimen testing, the public health unit and the home can determine the appropriate next steps to mitigate transmission by implementing targeted interventions.
Specimen collection kits (stool or NP) can be ordered through Ottawa Public Health using the Facility Stool Specimen Collection Request Form when an outbreak has been declared.
Kit contains two sterile containers:
Container Number | Colour of Cap | Transport Media | Test Required |
#1 |
White cap |
Empty vial |
Virology |
#2 |
Green cap |
Red transport medium |
Bacteriology |
Collection:
- Check the expiration date on the sterile containers. DO NOT use expired kits
- If blood or mucus is present in the stool, take the sample from this part of the stool
- Stool specimens that have been in contact with water in the toilet are unable to be accepted due to contamination
Collection Procedure:
*These first two steps are very important – if the label and/or form are missing information, illegible, or filled out incorrectly, the lab will not test the sample.
- Label the sample containers with the child’s name, health card number (ID number on the label), and the date/time the stool sample is collected.
- Complete all of the required sections of the “General Test Requisition” form
- Patient information section
- Public health unit outbreak number
- Date stool sample collected
- Date of symptom onset (date symptoms started)
- Collect the stool sample
- Adults and toilet trained children: Defecate into a clean container (i.e. a disposable plate). Stool that has been in contact with water is cannot be accepted. TIP: Urinate first if needed.
- Children who are not toilet trained: Collect the stool sample from the soiled diaper or directly from the child's training "potty."
- Using the individual "scoop" attached to each of the caps, transfer stool into the corresponding sterile containers. Fill the white cap container first.
- Fill to the line indicated on the container. Do not overfill.
- Tighten the caps. Do not use excessive force to tighten the caps as the containers may crack and leak.
- Place the containers in the inner pocket of the plastic biohazard bag and seal the zip-lock bag.
- Place the General Test Requisition form in the small outer pocket of the plastic biohazard bag. Do not place the form in the inner pocket.
- Place the biohazard bag into the brown paper bag and refrigerate the sample(s) immediately after collection. Stool samples should be placed on the bottom shelf of a fridge and food should not be stored in the same fridge as the stool samples. A cooler with ice or an ice pack can be used if a separate fridge is not available. Do not freeze the sample.
- Perform hand hygiene. If hands are visibly soiled, use soap and water.
- Call Ottawa Public Health at 613-580-2424, ext. 26325 for stool sample pick-up and delivery to the Public Health Laboratory located in Ottawa. Deadline for same day pick up is 3:30 pm. Inform your child care and early years operator that a stool sample was collected.
Labelling:
Label each container with the client's name, Date of Birth and date the stool sample was collected. Samples that are incorrectly filled out, or packaged improperly will be discarded by Public Health Lab.
For resources related to communication about enteric illness, please refer to the following documents:
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Shared items and high touch surfaces can be sources for spread of infection, especially in child care settings and schools. Such surfaces may include door knobs, taps, handrails, phones, computer keyboards, elevator buttons, countertops, as well as shared sports equipment or toys. These surfaces and items can easily become contaminated by viruses or bacteria which can spread from one person to another. Developing policies and procedures for cleaning and disinfection is important to prevent the spread of infections in your setting or school.
Cleaning
Cleaning is the physical action of removing debris from a surface. Cleaning with soap or detergent, and water, will remove organic material such as food, body fluids, fecal matter, or soil from surfaces. Residue from the soap or detergent must be rinsed off prior to disinfection to prevent neutralization of some disinfectants.
Disinfection
Disinfection must occur after cleaning. Disinfection inactivates or kills microorganisms (germs) that may be present on surfaces and that cleaning does not remove. All-in-one cleaning/disinfecting products must contain a drug identification number (DIN) from Health Canada. The disinfectant contact time, which is the time that a surface must remain wet for a disinfectant to be effective, varies between products. Follow manufacturers' instructions for disinfectant contact times, dilution and handling of products, including for pre-saturated disinfectant wipes. Flip-top bottles are preferred to apply cleaning solution. If using a spray bottle, saturate the cloth first then wipe the surface. Avoid the use of aerosol cans as this can propel the germs and cleaning solution into the air. Household bleach diluted in water is an inexpensive and effective disinfectant and can be used for most surfaces. It should be prepared daily. Ensure that surfaces are cleaned with a detergent or cleaning agent before the household bleach mixture is used as a disinfectant. Mixing some cleaners and disinfectants (like chlorine bleach and ammonia) can be harmful, even deadly. Others can irritate your eyes, nose, or throat and cause breathing problems.
Guide for preparation of bleach solutions |
The following table can be used as a reference for preparing household bleach solutions:
100 parts per million (ppm)
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To sanitize items such as combs, brushes and floors
To sanitize kitchen utensils in a commercial dishwasher
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2 mL of bleach with 1 litre of water
OR ½ teaspoon (tsp) of bleach with 4 cups of water
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1 minute
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200 ppm
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To sanitize dishes and utensils in place when they are too large to be washed in a dishwasher or submerged in a sink
To sanitize kitchen surfaces such as counter tops and cutting boards
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4 mL of bleach with 1 litre of water
OR
¾ tsp of bleach with 4 cups of water
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1 minute
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500 ppm
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To sanitize child care surfaces, diapering stations, toys, play areas, pet cages and high touch surfaces
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10 mL of bleach with 1 litre of water
OR
2 tsp of bleach with 4 cups of water
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2 minutes
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1000 ppm
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During outbreaks, to sanitize child care surfaces, diapering stations, toys, play areas, pet cages and high touch hard surfaces.
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20 mL of bleach with 1 litre of water
OR
4 tsp of bleach with 4 cups of water
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1 minute |
5000 ppm
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To disinfect surfaces or items that have been in contact with blood or body fluids, including blood spills, vomit or fecal (stool) contamination
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100 mL of bleach with 1 litre of water
OR
½ cup of bleach with 4 cups of water
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≥10 minutes
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*Note: The contact time, also known as the wet time, is the time that the disinfectant needs to stay wet on a surface to make sure it can kill all the germs. It is the length of time you leave the solution on the surface before wiping it down.
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Cleaning and disinfection checklist
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Commonly touched surfaces and items should be cleaned and disinfected frequently (e.g. at least once a day) to limit the potential spread of germs. When the setting is experiencing an outbreak, cleaning and disinfection of these surfaces and items is required more often. Develop a checklist specific to your facility, using this checklist as a guide. Remember to always work from clean areas to dirty areas.
General areas:
- Door knobs/frames/handles/other frequently touched areas on the door
- Locker doors/cubby holes/storage bins for children
- Desks/tables/chairs/counter tops
- Hand railings
- Light switches
- Water fountains
- Elevator buttons
- Shared equipment (such as toys, sports equipment and musical instruments after each use, computer keyboards and mice, etc.)
- Telephones/faxes/photocopiers/intercoms
- Play areas and toy storage areas
- Floors (especially where children play on them)
Kitchen/staff room:
- Food preparation areas and equipment
- Counter tops
- Fridge handles
- Microwaves
- Coffee pots/kettles
- Tables/chairs
Child and staff washrooms:
- Stall door edges and locks
- Coat hooks
- Sanitary napkin dispensers
- Paper towel dispensers
- Soap dispensers
- Taps/faucets
- Flush handles
- Toilet seats
- Toilet bowls and urinals
Important points to remember:
- Use single use cloths or disposable paper towels to clean sinks and toilets/urinals
- Ensure an adequate supply of paper towels and soap
- Do not top up partially empty dispensers; once empty, containers should be cleaned, disinfected, dried, and then refilled
- Soap/detergent must be rinsed off prior to disinfection
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Cleaning and Disinfection of Toys
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Infants and young children share toys and often place them in their mouths. This increases the risk of spreading infections. To reduce this risk, play areas and storage spaces should be cleaned and disinfected on a regular basis. Ensure that the disinfectant is safe and suitable for use on toys. Consult the manufacturer's recommendations for dilution and contact times for these disinfectants.
Cleaning and disinfecting hard plastic toys
- Remove toys from the play area after use and place them in a collection box for cleaning and disinfection
- Clean and disinfect the collection box at the same time that toys are being cleaned and disinfected
- Clean toys in hot soapy water prior to using a disinfectant
- Use a brush to clean crevices or hard to reach areas
- Rinse toys well under running water as soap may neutralize the disinfectant
- Soak toys in an appropriate disinfectant for required amount of time (contact time)
- Rinse toys with clean water to remove any disinfectant solution, if indicated on the label; bleach does not require rinsing
- Clean and disinfect dishwasher-safe, hard plastic toys in a commercial dishwasher with a sanitizer or a hot rinse cycle
- Completely air dry toys before they are returned to use
- Keep a record of when toys were cleaned and disinfected
Cleaning and disinfecting soft, porous toys or dress up clothes
- Launder fabrics or plush toys in a washing machine with hot water, and dry in a clothes dryer on a hot cycle
- Avoid using disinfectant products on porous surfaces
Cleaning other items
- Clean and disinfect other items (e.g., scissors, puzzles, storage bins, etc.) when they are visibly dirty
- Items such as books and some craft equipment may be difficult to clean, so consider discarding them once they are soiled
- Avoid sensory play during an outbreak, such as activities using play dough, sand or water
- Clean and disinfect computer keyboards, mice and other electronics between uses
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Diaper changing
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Diapering areas in child care settings are important to consider in preventing the spread of germs.
The following measures are important infection prevention and control practices for such areas:
- Keep the diapering stations separate from food preparation, eating and activity areas
- Ensure the surface for diapering is made of a smooth, non-porous and cleanable material
- Dedicate a hand wash sink close to the diapering station with warm water, soap and disposable towels
- When diapering a child, it is important to ensure that staff wash their hands prior to putting on gloves and handling the soiled diaper
- Gloves should be removed after handling the soiled diaper, followed by hand washing
- Gloves are single use and should not be used for another task or for diapering another child
- Place soiled diapers immediately in a nearby garbage can with a secure lid and disposable liner; a foot operated garbage can is preferred
- Used cloth diapers (outer covering and liner) must be handled as little as possible to limit and prevent exposure to body fluids. Do not shake, scrape, or rinse the cloth diapers. However, formed stool may be released into a toilet if it can be done so with ease, then place soiled items into a plastic bag and return to parents. During an outbreak, children using cloth diapers should switch to disposable diapers for the duration of the outbreak
- Ensure personal diapering items are clearly labelled for each child (creams, wipes and diapers); do not use a child's dedicated items on another child
- If a child's clothing becomes soiled, place it in a plastic bag and send home for laundering
- Use an appropriate cleaner and disinfectant to clean the diapering surface between each use (e.g., after each diaper change)
- Assist children with hand washing after diapering
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